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<t>α2M</t> level decreased in degenerated NP tissue. Representative images of (A) MRI, the yellow arrows indicated the operation section, (B) HE staining (magnification: 200×) (C) IHC targeting α2M of both mildly and severely degenerated NP tissue. (magnification: 200×) (D) Quantification analysis of IHC. NP tissue from the 16 patients was lysed to measure (E) α2M with ELISA methods and (F) total ROS level. The values are mean ± SD of three independent experiments. (***P<0.001)
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α2M level decreased in degenerated NP tissue. Representative images of (A) MRI, the yellow arrows indicated the operation section, (B) HE staining (magnification: 200×) (C) IHC targeting α2M of both mildly and severely degenerated NP tissue. (magnification: 200×) (D) Quantification analysis of IHC. NP tissue from the 16 patients was lysed to measure (E) α2M with ELISA methods and (F) total ROS level. The values are mean ± SD of three independent experiments. (***P<0.001)

Journal: Journal of Medical Biochemistry

Article Title: Antioxidative behavior of a2-macroglobulin in intervertebral disc degeneration

doi: 10.5937/jomb0-39557

Figure Lengend Snippet: α2M level decreased in degenerated NP tissue. Representative images of (A) MRI, the yellow arrows indicated the operation section, (B) HE staining (magnification: 200×) (C) IHC targeting α2M of both mildly and severely degenerated NP tissue. (magnification: 200×) (D) Quantification analysis of IHC. NP tissue from the 16 patients was lysed to measure (E) α2M with ELISA methods and (F) total ROS level. The values are mean ± SD of three independent experiments. (***P<0.001)

Article Snippet: Additionally, α2M from human plasma (Sigma-Aldrich, St. Louis, MO, USA) was used to reverse HOCl.

Techniques: Staining, Enzyme-linked Immunosorbent Assay

α2M stimulation decreased ROS, MMP13, and ADAMTS4 of severely degenerated NP tissue. We cultured severely degenerated NP tissue in α2M (50, 150, 200 nmol/L) growth medium for 3 days. The protein expression level of α2M was determined by (A) IHC (magnification: 200×) and (B) quantification analysis. (C) Total ROS level of NP tissue. (D) The content of MMP13 and ADAMTS4 was assayed by ELISA. The values are mean ± SD of three independent experiments. (*P<0.05, **P<0.01)

Journal: Journal of Medical Biochemistry

Article Title: Antioxidative behavior of a2-macroglobulin in intervertebral disc degeneration

doi: 10.5937/jomb0-39557

Figure Lengend Snippet: α2M stimulation decreased ROS, MMP13, and ADAMTS4 of severely degenerated NP tissue. We cultured severely degenerated NP tissue in α2M (50, 150, 200 nmol/L) growth medium for 3 days. The protein expression level of α2M was determined by (A) IHC (magnification: 200×) and (B) quantification analysis. (C) Total ROS level of NP tissue. (D) The content of MMP13 and ADAMTS4 was assayed by ELISA. The values are mean ± SD of three independent experiments. (*P<0.05, **P<0.01)

Article Snippet: Additionally, α2M from human plasma (Sigma-Aldrich, St. Louis, MO, USA) was used to reverse HOCl.

Techniques: Cell Culture, Expressing, Enzyme-linked Immunosorbent Assay

α2M stimulation reversed HOCl-induced oxidative stress of NP cells. NP cells of mildly degenerated NP tissue were treated with HOCl (from 10 to 50 μmol/L) for 6 h, or treated with 30 μmol/L from 3 h to 24 h; Besides, NP cells were pretreated with 30 μmol/L HOCH for 6 h and then cultured with α2M (from 50 to 200 nmol/L) for another 24 h, or cultured with 200 nmol/L α2M from 3 h to 24 h. (A, B) The protein expression level of α2M was determined by ELISA. (C, D) Total ROS level of NP cells. The values are mean ± SD of three independent experiments.

Journal: Journal of Medical Biochemistry

Article Title: Antioxidative behavior of a2-macroglobulin in intervertebral disc degeneration

doi: 10.5937/jomb0-39557

Figure Lengend Snippet: α2M stimulation reversed HOCl-induced oxidative stress of NP cells. NP cells of mildly degenerated NP tissue were treated with HOCl (from 10 to 50 μmol/L) for 6 h, or treated with 30 μmol/L from 3 h to 24 h; Besides, NP cells were pretreated with 30 μmol/L HOCH for 6 h and then cultured with α2M (from 50 to 200 nmol/L) for another 24 h, or cultured with 200 nmol/L α2M from 3 h to 24 h. (A, B) The protein expression level of α2M was determined by ELISA. (C, D) Total ROS level of NP cells. The values are mean ± SD of three independent experiments.

Article Snippet: Additionally, α2M from human plasma (Sigma-Aldrich, St. Louis, MO, USA) was used to reverse HOCl.

Techniques: Cell Culture, Expressing, Enzyme-linked Immunosorbent Assay

α2M level decreased in degenerated NP tissue. Representative images of (A) MRI, the yellow arrows indicated the operation section, (B) HE staining (magnification: 200×) (C) IHC targeting α2M of both mildly and severely degenerated NP tissue. (magnification: 200×) (D) Quantification analysis of IHC. NP tissue from the 16 patients was lysed to measure (E) α2M with ELISA methods and (F) total ROS level. The values are mean ± SD of three independent experiments. (***P<0.001)

Journal: Journal of Medical Biochemistry

Article Title: Antioxidative behavior of a2-macroglobulin in intervertebral disc degeneration

doi: 10.5937/jomb0-39557

Figure Lengend Snippet: α2M level decreased in degenerated NP tissue. Representative images of (A) MRI, the yellow arrows indicated the operation section, (B) HE staining (magnification: 200×) (C) IHC targeting α2M of both mildly and severely degenerated NP tissue. (magnification: 200×) (D) Quantification analysis of IHC. NP tissue from the 16 patients was lysed to measure (E) α2M with ELISA methods and (F) total ROS level. The values are mean ± SD of three independent experiments. (***P<0.001)

Article Snippet: Additionally, α2M from human plasma (Sigma-Aldrich, St. Louis, MO, USA) was used to reverse HOCl.

Techniques: Staining, Enzyme-linked Immunosorbent Assay